Richard Greene

Sir Henry Baskerville

Basil Rathbone

Sherlock Holmes

Wendy Barrie

Beryl Stapleton

Nigel Bruce

Doctor John H. Watson

Lionel Atwill

Dr. James Mortimer

John Carradine

Barryman

Barlowe Borland

Frankland

Beryl Mercer

Mrs. Jennifer Mortimer

Morton Lowry

John 'Jack' Stapleton

Ralph Forbes

Sir Hugo Baskerville

E. E. Clive

London Cabbie John Clayton

Eily Malyon

Mrs. Barryman

Lionel Pape

The Coroner

Nigel De Brulier

The Notting Hill Murderer

Mary Gordon

Mrs. Hudson

Ian Maclaren

Sir Charles Baskerville

Ruth Terry

Betsy Ann

Mary Young

Betsy Ann's Mother (uncredited)

Kenneth Hunter

Ship's Officer (uncredited)

Vesey O'Davoren

Ship's Steward (uncredited)

John Graham Spacey

Ship's Porter (uncredited)

David Thursby

Open Carriage Driver (uncredited)

Peter Willes

Roderick

Leonard Carey

Hugo's Servant (uncredited)